No 6, Caotang Industry Park, No.2 Qinling Road, Hi-tech Zone, Xi'an, China
b. Sartorius electronic balance(Germany)
c. KQ-----model 100, Washing device of ultrasonic wave 100-KQ
Chromatographic Column ќ ROM-150 ODS-c18(4.6mm×250mm, 5ц)
Mobile Phase: methanol : Water：Acetic Acid =80:20：0.1
Wave Length: 254nm
Column: room temperature：25℃
Transfer about 5 mg sample of aloe-emodin standard powder , accurately weighed, to a 25mL volumetric flask, add the right amount methanol to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the methanol solution. Then add water to scale mark, it is obtained.
Transfer 5 mg sample of aloe-emodin powder, accurately weighed, to a 25ml volumetric flask, add the right
amount methanol to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the methanol, Then add methanol to scale mark,shake evenly, it is obtained.
Separately inject equal volumes (20uL) of the Standard preparation and the Assay preparation into the chromatograph, record th chromatograms, and measure the responses for the major peaks.
6. Content calculation