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Test Method of Physcion by HPLC
Aug 26, 2017

Rhubarb is a group of plants that belong to the genus Rheum. The active ingredient of rhubarb is chrysophanol / chrysophanic acid and physcion(also named parietin).

English name:            Physcion (Rhubarb Extract )

Plant original:             Rheum palmatum L.

Other name:               Parietin

Specification:              Physcion 10%, 20%,40%,95%,98%


Determination of Physcion Content


Chromatographic condition and system applicability test:

Mobile Phase: Methanol: water: glacial acetic acid =79.5 :19.5 : 1

Wave Length: 254nm

Chromatographic Column: C18 (4.6MM × 150mm , 5μm)

(According to the test method of emodin in Chinese Pharmacopoeia 2005 )

 

Standard solution of Physcion


Weight accurately 2mg physcion, add to a 25mL volumetric flask, add the right amount acetone to dissolve, Ultrasonic wave is shaken till all dissolve in the acetone. Then add acetone to scale mark, obtained 5 standard solution.

 

Testing solution of Physcion


Weight accurately 10mg physcion sample, to a 25ml volumetric flask, add the right amount acetone to dissolve, Ultrasonic wave is shaken till all dissolve in the acetone, Then add acetone to scale mark, shake evenly, it is obtained.

 

Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks.

          A1    W2

X%=----- × ----  × 100%

         A2     W1

In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1  is the concentration of assay sample.


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