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Test Method of Hydroxyanthracenes in Rheum extract by HPLC
Oct 21, 2017

Determination of Content

Chromatographic condition and system applicability test:

Mobile Phase: Methanol: water: glacial acetic acid =79.5 :19.5 : 1

Wave Length: 254nm

Chromatographic Column: C18 (4.6MM × 150mm , 5μm)

(According to the test method of emodin in Chinese Pharmacopoeia 2005 )


Standard solution

Weight accurately 2mg Emodin standard, Rhein standard, aloe-emodin standard, chrysophanol standard and physcion standard Separately, separately add to a 25mL volumetric flask, add the right amount  acetone to dissolve, Ultrasonic wave is shaken till all dissolve in the acetone. Then add acetone to scale mark, obtained 5 standard solution.


Testing solution

Weight accurately 10mg rheum extract sample, to a 25ml volumetric flask, add the right amount acetone to dissolve, Ultrasonic wave is shaken till all dissolve in the acetone, Then add acetone  to scale mark, shake evenly, it is obtained.


Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks.

         A1    W2

X%=----- × ----  × 100%

         A2     W1

In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1  is the concentration of assay sample.


Calculate the content of Hydroxyanthracenes:

Add the following five active ingredients, obtained the content of Hydroxyanthracenes.

1.     Emodin

2.     Rhein

3.      Aloe emodin

4.      Chrysophanol

5.      Physcion

Where to buy Rheum Extract?

Xi'an Natural Field Bio-Technique Co.,Ltd. as Rheum extract HPLC suppliers, which is the professional Manufacturer of Herbal Extract Since 2005. And attend the CPHI, Vitafoods and API Exhibition from 2008. Hydroxyanthracenes is the one of strong products, we will supply Rheum extract (Emodin,Rhein,Aloe emodin,Chrysophanol,physcion) and service to you. the Rheum extract are widely used for health care foods and pharma field. If you need this product and related product, please contact us or send an inquiry to [email protected] at any time.

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