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Test method of Amygdalin
Mar 24, 2017

Test method of Amygdalin

1. Instrument

a. HPLC-9a (Shimadzu)    

b. Sartorius electronic balance (Germany)

c. KQ-----model 100, Washing device of ultrasonic wave


2. Condition

Chromatographic Column ќ ROM-150 ODS-c18(4.6mm×150mm, 5ц)

Mobile Phase: Acetonitrile:Water=20∶80 (V/V)

Flow Rate: 1.0mL/min

Wave Length: 220nm

Column: room temperature


3. Standard solution

Transfer about 8 mg amygdalin standard( Medicine Institute company of CHINA), accurately weighed, to a 25mL volumetric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water. Then add water to scale mark, it is obtained.


4. Testing solution

Transfer 8mg amygdalin powder, accurately weighed, to a 25ml volumetric flask

, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water, Then add water  to scale mark, shake evenly, it is obtained.


5. Procedure

Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks.         

      A1    W2

X%=----- × ----  × 100%

      A2     W1

In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1  is the concentration of assay sample.

      A1    W2

X%=----- × ----  × 100%

      A2    W1



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