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Method of Analysis——Formononetin
Sep 28, 2017

Xi'an Natural Field Bio-Technique Co.,Ltd. owned stardard testing room. All subterranean clover formononetin testing method of Formononetin NMR(HNMR), Formononetin HPLC, Formononetin TLC, Formononetin UV, Formononetin PH Meter, Formononetin Moisture can be operated by our customers. Effect of formononetin on osteoblasts is obvious, we can supply the free sample 10g for your testing. At present, share test method of formononetin to you as following:



1. Instrument    设备

a HPLC-9a (Shimadzu)   

b Sartorius electronic balance (Germany) 电子称

c. KQ-----model 100, Washing device of ultrasonic wave  100-KQ 超声波洗涤设备

 

2. Condition 条件

Chromatographic Column 色谱柱Inertsil   ODS -34.6*150mm   5um

Mobile Phase: Methyl alcohol : Acetonitrile: Water:Acetic acid =30∶38:60: 0.1

流动相:甲醇:乙腈:水:磷酸(30:38:60:0.1)

Flow Rate: 1.0mL/min  流速

Wave Length: 262nm   波长

Column: room temperature 测柱: 室温


3.Standard solution  标准溶液

Transfer about 5mg Formononetin standard(Sigma company U.S.A.), accurately weighed, to a 25mL volumetric flask, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water. Then add water to scale mark, it is obtained.

 

4.Testing solution 测试溶液

Transfer 5mg Formononetin powder, accurately weighed, to a 25ml volumetric flask

, add the right amount water to dissolve, Ultrasonic wave is shaken for 5 minutes till all dissolve in the water, Then add water to scale mark, shake evenly, it is obtained.

 

5. Procedure 过程

Separately inject equal volumes (about 20 uL) of the Standard preparation and the Assay preparation into the chromatograph, record the chromatograms, and measure the responses for the major peaks.        

        A1    W2

X%=----- × ----  × 100%

        A2     W1

In which is A1 the assay sample , A2 is the standard peak area, W2 is the concentration of standard, W1  is the concentration of assay sample.


Where Can I Buy Formononetin?

Xi'an Natural Field Bio-Technique Co.,Ltd. as Formononetin Manufacturer, which is the professional Manufacturer of Herbal Extract Since 2005. And attend the CPHI, Vitafoods and API Exhibition from 2008. formononetin biosynthesis 98% and Natural (sources of formononetin)Red Clover Extract Formononetin 98% are the strong products, we will supply the best quality supplement price and service to you. If you need subterranean clover formononetin and related product, please contact us or send an inquiry to info@natural-field.com at any time.