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How is NMN made?
Nov 18, 2021

The importance of NMN turned out to be more and more important, so how did it synthesize it? The latest known methods for chemical synthesis of NMN are gradually improved under the continuous efforts of different researchers. There are mainly two methods as follows.

  1. Using tetraacetyl ribose and ethyl nicotinate as raw materials

Using tetraacetyl ribose and ethyl nicotinic acid as raw materials, they are condensed under the condition of trimethylsilyl trifluoromethanesulfonate (TMSOTf) to form compound 3, which is then removed under the condition of sodium ethoxide Acetyl protection gave compound 4, followed by phosphorylation with phosphorus oxychloride to give compound 5, and finally amination with ammonia to obtain the target product NMN. In this route, the first two steps do not require purification to obtain NMR compound 4 without obvious impurities, and the yield is about 80%, and only the most The latter step needs to be purified by ion exchange resin to remove inorganic salts, such as ammonium chloride, etc., acidified to remove excess ammonia, and then HPLC can be obtained. Β-NMN with a purity of more than 97%.


2.Using diphenyl chlorophosphate as phosphorylation reagent.

The first step is the esterification reaction. Acetonitrile

As a solvent, pyridine is used as an acid binding agent, compound 6 (β-nicotinamide ribose) and compound 7 (diphenyl chlorophosphate) undergo an esterification reaction to produce compound 8. After removing the solvent, it is directly used in the next step without purification; The second step is the hydrolysis reaction. Through metal-catalyzed hydrogenation reaction Β-NMN, after removing the catalyst and solvent, through ion exchange After the resin is purified, β-NMN with a purity of 99.3% can be obtained, and the yield is about 64%.


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